That the rich puppet tradition in Pilsen has not died the visitors can satisfy themselves in the room devoted to the ensembles of the present and recent periods. The ensembles represented in the Museum are Špalíček, V Boudě, Střípek, Rámus and Divadélko dobrý den.

The present professional Theatre Alfa with its puppets from numerous prized author performances presents itself on the third floor. In addition to the traditionally conceived exhibition areas, the Museum also offers a room where visitors can handle puppets and familiarize with the various puppet-leading techniques. This multi-purpose hall also serves as a place where theatre performances, lectures or conferences are organized.